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| Optical Sectioning with the ApoTome |
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| The Innovative Dimension in Fluorescence Microscopy |
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Target group
Microscopists dealing with fluorescent biomedical specimens
Prior skills required
Fluorescence microscopy (e.g., Course on "Optimized Fluorescence Microscopy")
Photomicrographs of thin fluorescent specimens are of outstanding brilliance. Many fluorescent objects, though, are too thick to yield well-defined, high-contrast images with conventional microscopes. The ApoTome creates ultrathin optical sections of thick or overstained objects. Stacks of such sections provide fluorescent images that are sharp and rich in contrast. The image stacks can also be assembled into 3D views. Thanks to their high contrast, ApoTome images show an amazing lot of detail.
The course will teach you how to use the ApoTome with your specimens in the most efficient way.
- Operating principle of the ApoTome
- Calibration and setting of the ApoTome
- Acquisition of multichannel fluorescent images
- Creation of Z image stacks and 3D views
- Comparison of methods to create optical sections
Duration
Two days, 9 a.m.- 4 p.m.
Participants
6 | |
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