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| Dynamic Live Cell Studies |
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| FRAP, FLIP, FRET, Photoactivation and Photoconversion |
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Prerequisites:
Basic knowledge in Laser Scanning Microscopy is recommended.
Beside their classical function as superior 2D and 3D imaging systems, advanced laser scanning microscopes can be used for studying dynamic and binding properties of biomolecules.
In dynamic live cell studies the laser is no longer just a source for excitation light but a precise tool for bleaching, photoactivation and photoconversion of fluorescent dyes in subcellular compartments. Get familiar with these innovative experimental approaches during this course, to apply them succesfully in your own work.
- FRAP, FLIP and FRET in Laser Scanning Microscopy: Theoretical principles
- Photactivatable GFP and Kaede as new, innovative tools in dynamic live cell studies
- Integration of bleaching experiments into time series
- FRET: Sensitized emission and acceptor bleaching
- FRAP and FLIP: System optimization for fast data acquisition
- Localized photoactivation and photoconversion with the blue diode laser
Duration:
two days, 9 a.m. – 6 p.m.
Participants:
6 | |
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